Products

SARS-CoV-2 pseudoviruses

SARS-CoV and SARS-CoV-2 S protein pseudotyped lentiviruses

We help our customers to assemble SARS-CoV and SARS-CoV-2 S protein pseudotyped lentiviruses. These particles carry reporters that can be used for antiviral drug screening or the quantification of neutralizing antibodies. Customers can provide us with the reporter construct of your choice, and we will assemble particles within a week. Please contact us by email: info@virongy.com

We also have several pre-assembled, concentrated (60 x concentrated) particles that are available for your initial testing. These particles are provided with a free sample of our proprietary SARS-CoV-2 Pseudovirus Infection Enhancer (CoV-2-PIE, Cat# CoV2-01) that can greatly promote productive viral infection. CoV-2-PIE can be used to facilitate the infection of a variety of host cells. CoV-2-PIE can enhance viral infection rates by 3 to 20 fold.

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SARS-CoV-2 Pseudovirus Infection Enhancer (CoV-2-PIE)

A cutting-edge technology to facilitate your COVID-19 research

CoV-2-PIE is a viral infection enhancer designed specifically to facilitate coronavirus and pseudovirus infection of target cells. CoV-2-PIE is formulated based on our current technology of Infectin-2TM, which is designed to penetrate the cortical actin barrier, thereby greatly enhancing productive viral infection. CoV-2-PIE can be used to facilitate the infection of a variety of host cells by coronaviruses and pseudoviruses. CoV-2-PIE can enhance viral infection rates by 5 to 20 fold. Virongy developed CoV-2-PIE based on the scientific theory that the actin cytoskeleton is a natural barrier for viral entry and post-entry intracellular migration (Yoder et al., Cell, 2008, 134:782). CoV-2-PIE is formulated to mainly enhance viral infection of adherent target cells, which are frequently also limited by poor absorption of virion particles to target cells. CoV-2-PIE combines our proprietary technology of InfectinTM with additional technologies that promote viral absorption onto target cells.

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Infectin-2TM

A cutting-edge technology in virology research
InfectinTM is a viral infection enhancer designed to facilitate viral penetration of the cortical actin barrier, thereby greatly enhancing productive viral infection. InfectinTM can be used to facilitate the infection of a variety of host cells by different viruses and viral vectors. InfectinTM can enhance viral infection rates by 5 to 20 fold. Virongy developed InfectinTM based on the scientific theory that the actin cytoskeleton is a natural barrier for viral entry and post-entry intracellular migration (Yoder et al., Cell, 2008, 134:782). Infectin-2TM is our second generation of InfectinTM that is formulated mainly to enhance viral infection of adherent cells, which is frequently also limited by poor absorption of virion particles to target cells. Infectin-2TM combines our proprietary technology of InfectinTM with additional technologies that promote viral absorption onto target cells.

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InfectinTM

Infectin is a viral infection enhancer designed to facilitate viral penetration of the cortical actin barrier, thereby greatly enhancing productive viral infection. Infectin can be used to facilitate the infection of a variety of host cells by different viruses and viral vectors. Infectin can enhance viral infection rates by 5 to 20 fold. Virongy developed Infectin based on the scientific theory that the actin cytoskeleton is a natural barrier for viral entry and post-entry intracellular migration.

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HIV Rev-dependent Reporter Cells

The HIV Rev-dependent reporter cells represent a major advancement in the development of HIV indicator cells. Our Rev-dependent reporter cells use both LTR and the Rev/RRE interaction to regulate reporter gene expression. This strict requirement for Rev, a viral protein present only in infected cells, drastically improves the reporter specificity and sensitivity. As a result, our Rev-dependent reporter cells are suitable for a broad range of applications, including screening broadly neutralizing antibodies and anti-HIV drugs, and studying HIV cell-cell transmission and host restriction and dependency factors. Derived from CD4 T cells, our reporter cells express native levels of HIV receptors and are natural HIV targets with broad susceptibility to X4, R5, primary HIV isolates, and certain SIV strains. With GFP, Luc, or GFP/Luc detection options, our Rev-dependent cells provide a versatile and flexible platform for HIV research.

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HIV InfectinTM

HIV InfectinTM is designed to facilitate HIV penetration of the cortical actin barrier, thereby greatly facilitating productive viral infection. HIV InfectinTM can be used to enhance the infection of a variety of host cells by HIV (both X4 and R5 viruses) to facilitate biochemical and biological characterization of HIV infection. HIV InfectinTM can enhance HIV infection rate 5 to 20 fold. Virongy developed HIV InfectinTM based on the scientific theory that the actin cytoskeleton is a natural barrier for HIV entry and intracellular migration.

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Intracellular Protein Staining Kit

Virongy’s Intracellular Protein Staining Kit provides a flexible and reliable tool for immunofluorescent staining of intracellular host and viral proteins, such as host phospho-proteins and viral capsid proteins. After staining, cells are ready for fluorescence-activated cell sorting and analysis. Virongy’s intracellular staining kit is compatible with various combinations of antibodies and cytokines to accommodate your individual research needs.

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LIM Domain Kinase (LIMK) Inhibitor R10015

LIM domain kinases (LIMKs) are serine/threonine and tyrosine kinases which phosphorylate and inactivate the ADF/cofilin family of proteins through serine 3 phosphorylation. The LIMK inhibitor R10015 is the best biochemically and biologically characterized LIMK inhibitor. R10015 has been profiled against a panel of 62 kinases. It directly inhibits the kinase activity of LIMK by binding to the ATP-binding pocket of LIMK. The in vitro IC50 value for inhibiting recombinant LIMK1 protein was approximately 38 nM. R10015 blocks the phosphorylation of cofilin at serine 3 in human T cells. R10015 inhibits chemokine-induced actin polymerization and T cell chemotaxis and. In addition, R10015 has been shown to possess broad-spectrum anti-viral activities as LIMK is involved in the infection and pathogenesis of multiple viruses such as HIV, influenza A, pseudorabies virus, EBOV, RVFV, VEEV, and HSV-1.

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Vader-Trap High-Purity Plasmid DNA Purification Kit

Virongy’s Vader-Trap High-Purity Plasmid DNA Purification Kit is built upon our proprietary technology. Our Vader-Trap plasmid purification technology significantly improves the traditional alkaline lysis procedure for plasmid purification. Virongy’s Vader-Trap technology uses innovative absorption particles and reagents to adsorb and remove contaminants and impurities, resulting in higher yield and greater purity than conventional plasmid DNA purification kits. The purified plasmid DNA is suitable for many downstream applications, such as restriction digestion, transfection, cloning, and DNA sequencing, all with greater efficiency.

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