Low-Speed Viral Concentration Kit

Product Description

Low-Speed Viral Concentration Kit

Virongy’s Low-Speed Viral Concentration Kit is designed to enhance virus titer, maximize transduction efficiency and increase particle purity. Our propriety Viral Concentration and Resuspension Buffers come sterile and ready-to-use for concentrating viruses, pseudoviruses, nanoparticles (50-200nm) and extracellular vesicles. The Viral Resuspension Buffer is cell culture and animal safe, for research purposes only. The concentrated viral particles have up to 10x the transduction efficiencies and the higher purity enhances cryo-electron microscopy imaging. The low-speed protocol does not require an ultra-centrifuge and can be scaled to any size of particle preparation.

Applications:

• Enhances viral titer
• Improves gene transduction efficiency
• Clears away debris for higher-quality particle EM imaging

Example Data:

GFP expressing VSV-G pseudotyped lentivirus were concentrated with the low-speed viral concentration kit and then used to infect HEK293T cells

HIV infectious units were quantified by Rev-A3R5.7-GFP cells following concentration with the low-speed viral concentration kit.

This easy-to-use, low-speed concentration kit allows users to concentrate viruses, pseudoviruses, and exosomes with just a 10000xg centrifuge. Simply add Virongy’s sterile, proprietary viral concentration buffer to a centrifuge tube, apply your virus-containing medium on top, and spin for four hours at 10,000xg. Concentrate up to 100X the original volume.

View Protocol