$750.00$14,100.00

Rapid Alpha-Pseudoviruses for Henipavirus

Nipah virus (NiV) pseudovirus based on NCBI Accession# NC_002728.1

In stock
Henipavirus Species Nipah Virus
Reporter Gene Firefly Luciferase
Size and Price 5 x 200 µL ~20 wells/96 well plate, 10 x 200 µL ~40 wells/96 well plate, 25 x 200 µL ~100 wells/96 well plate, 50 x 200 µL ~200 wells/96 well plate, 100 x 200 µL ~400 wells/96 well plate
N/A , , , , , ,

Product Description

Product Description

A rapid alpha-pseudovirus for Nipah and other Henipaviruses

Nipah virus (NiV) proteins in the alpha-pseudovirus are based on NCBI Accession# NC_002728.1

Applications

  • Rapid NiV pseudovirus transduction of target cells for viral entry and functional studies
  • Anti-NiV  drug screening
  • Anti-NiV neutralizing antibody screening

A novel rapid hybrid alpha-pseudovirus for Nipah virus (HA-NiV) and other Henipaviruses. HA-NiV particles are pseudoviruses assembled from the structural proteins of the Nipah virus, Fusion (F), Glycoprotein (G), Matrix (M), and Nucleocapsid (N) proteins and package an alphaviral vector for reporter gene expression.  The alpha-pseudoviruses are single-cycle viruses with self-replicating RNA for rapid quantification of neutralizing antibodies and entry-inhibiting drugs.  These pseudoviruses are BSL-2 safe and ready to use for studying viral entry. Additionally, we help our customers to assemble HA-NiV pseudoviruses at any scale.

If you have any additional questions please contact us by email: info@virongy.com

 

To enhance your pseudovirus entry, ask about receiving a free sample of our propriety Infectin that can significantly promote productive viral infection in a variety of host cells, enhancing viral infection rates by 3 to 20-fold.

Example of results:

HA-NiV particles are assembled with the NiV F, G, M, and N proteins and Virongy’s proprietary alphavirus vector genome. The alpha-pseudoviruses are used to transduce HEK293T cells (Left):

HEK293T cells were transduced with HA-NiV(Luc) pseudovirus (with a luciferase reporter). Reporter expression was quantified at 24hrs post-transduction (luciferase assay).

HA-NiV(GFP) transduction of HEK293T cells (Right):

HEK293T cells were transduced with HA-NiV(GFP) particles.  Reporter expression was quantified at 24 hours post-transduction.

 

HA-NiV and other alpha-pseudovirus particles are intended for Research Use Only and are not for diagnostic or therapeutic purposes or use in humans or animals.