SARS-CoV-2 Reporter Cell Line

Product Description

SARS-Cov-2 Reporter Cell Line

The SARS-CoV-2 reporter cells represent a major advancement in the development of rapid coronavirus indicators cells.  Our reporter cells carry a stably integrated construct that is derived from a mini-antisense genome of SARS-CoV-2. Authentic SARS-CoV-2 replicase proteins permit reporter expression by converting the mini-antisense genome into a positive-sense genome. The cell line is derived from Virongy’s HEK293T(ACE2/TMPRSS2) cells which express high levels of  ACE2 and TMPRSS2 to permit SARS-CoV-2 entry. The SARS-CoV-2 GFP reporter cells turn GFP positive upon live virus infection.  The SARS-CoV-2 reporter cell line does not produce any viral proteins and does not greatly impact overall viral replication.

Example Data:

SARS-CoV-2 Reporter Cell Line Using Fluorescent Microscopy

SARS-CoV-2 Reporter cells were infected with either mock virus or authentic SARS-CoV-2. The reporter was quantified by fluorescent microscopy two days post-infection

Key Features:

• High Sensitivity and Specificity – Minimal Background Signal
• Rapid quantification of SARS-CoV-2 infectious titer in less than 24 hours.
• No need for performing plaque assays and counting plaques
• GFP reporter for rapid and easy enumeration of infected cells with a flow cytometer or fluorescent microscope
• With enhanced accuracy – results are not affected by inaccurate plaque counting and loss of cells during cell fixing and staining.

Email info@virongy.com for academic pricing and special discounts