Filoviridae Pseudoviruses

Filoviruses include Marburg and Ebolaviruses. Marburg virus glycoprotein pseudotyped lentivirus is based on the MARV001 Isolate (Genebank: OL702894.1).  Ebolavirus glycoprotein pseudotyped lentiviruses based on either the Zaire ebolavirus (Genebank: KJ660346.2) or the Sudan ebolavirus (Genebank: MT583348.1) strain.

Applications:

• Marburg and Ebola pseudovirus transduction of target cells for viral entry and functional studies
• Anti-Marburg and Anti-Ebola drug screening
• Anti-Marburg and Anti-Ebola neutralizing antibody screening

We have developed several pre-assembled Marburg and Ebolavirus GP pseudotyped lentiviral particles that are available for your initial testing. These pseudoviruses are BSL-2 safe and ready to use for studying viral entry.  Additionally, we help our customers to assemble custom Marburg virus (MARV) and Ebolavirus (EBOV) Glycoprotein pseudotyped lentiviruses at any scale. These particles carry reporters that can be used for antiviral drug screening or the quantification of neutralizing antibodies. Customers can provide us with the reporter construct of their choice, and we will assemble particles within a week. Please contact us by email: info@virongy.com

To enhance your pseudovirus entry, ask about receiving a free sample of our propriety Infectin that can significantly promote productive viral infection in a variety of host cells, enhancing viral infection rates by 3 to 20-fold.

Background:

Ebolavirus is the causative agent of ebolavirus disease (EDV) characterized by severe hemorrhagic fever. EVD is a disease of human and non-human primates that is characterized by a high fatality rate (30–90%).  Ebolavirus is an enveloped, negative-stranded RNA virus characterized by a viron of ≈80 nm in diameter and a length ranging from hundreds of nanometers to micrometers. The genome encodes for seven structural proteins: the nucleoprotein (N), the virion protein (VP) 24, VP35, VP30, VP40, the glycoprotein (GP), and the RNA-dependent RNA polymerase (L). The virus enters the cell by fusion following the attachment of the viral Glycoprotein (GP) to the cell surface receptor Tim-1 and NPC1. This mechanism of entry allows for a broad host cell range.

Example of results:

Ebolavirus GP  pseudotyped lentiviral particle transduction of HEK293T cells (Left):

HEK293T cells were transduced with EBOV(Luc) lenti-pseudovirus (with a luciferase reporter). Reporter expression was quantified at 3 days post-transduction (luciferase assay).

Ebolavirus GP pseudotyped lentiviral particle transduction of HEK293T cells (Right):

HEK293T cells were transduced with EBOV(GFP) lenti-pseudovirus (with a GFP reporter).  Reporter expression was quantified at 2 days post-transduction (GFP flow cytometry).