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Rapid Alpha-Pseudoviruses for Arenaviruses

Name: Rapid Alpha-Pseudoviruses for Arenaviruses
SKU: 4884
Availability: InStock

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Reporter Gene	Firefly Luciferase
Size and Price	5 x 200 µL ~20 wells/96 well plate, 10 x 200 µL ~40 wells/96 well plate, 25 x 200 µL ~100 wells/96 well plate, 50 x 200 µL ~200 wells/96 well plate, 100 x 200 µL ~400 wells/96 well plate, 5 x 100 µL (Concentrated Only)

SKU:N/A Categories:Arenaviridae, Pseudovirus Tags:lassa pseudovirus, LASV, lenti-pseudovirus, lentivirus pseudotyped lassa, Pseudovirus

Product Description

Rapid Alpha-Pseudovirus for Lassa Virus

Lassa virus (LASV) Glycoprotein Complex (GPC) hybrid Alpha-pseudovirus, formulated using the NCBI reference sequence KU961971.1.

Applications:

Facilitating LASV pseudovirus transduction of target cells for viral entry and functional studies
Enabling rapid Anti-LASV drug screening
Supporting swift Anti-LASV neutralizing antibody screening

Description:

A novel rapid hybrid alpha-pseudovirus for Lassa virus (HA-LASV) and other Bunyavirales viruses. HA-LASV particles are pseudoviruses assembled from the structural proteins of the Lassa virus, glycoprotein (GP), and the Zinc-binding protein (Z) and package an alphaviral vector for reporter gene expression.

The alpha-pseudoviruses are single-cycle viruses with self-replicating RNA for rapid quantification of neutralizing antibodies and entry-inhibiting drugs. These pseudoviruses are BSL-2 safe and ready to use for studying viral entry. Additionally, we help our customers to assemble HA-LASV pseudoviruses at any scale.

To enhance your pseudovirus entry, ask about receiving a free sample of our propriety Infectin^TM which can significantly promote productive viral infection in a variety of host cells, enhancing viral infection rates by 3 to 20-fold.

Background:

Each LASV virion contains two single-stranded RNA segments, in which each segment encodes two proteins. The nucleoprotein (NP) encapsulates the viral genome segments, which is essential for both transcription of mRNA and replication of genome segments. The glycoprotein complex (GPC) mediates viral attachment and cell entry. The large (L) protein is an RNA polymerase involved in transcription, replication, and cap-snatching. The Zinc-binding (Z) protein serves as a matrix protein responsible for viral assembly and budding. Additionally, the Z protein negatively regulates viral replication and transcription, which is important for the suppression of viral and host cell translation.

LASV enters the host cell via receptor-mediated endocytosis, particularly, by binding to the peripheral membrane at the cell surface called α-dystroglycan (α-DG). As the endosomal pH drops, the LASV glycoprotein complex (GPC) undergoes a conformational shift that enables the dissociation from α-DG and binding to an endosomal receptor, lysosomal-associated membrane protein 1 (LAMP1). Once bound to LAMP1, the GPC undergoes additional conformational changes that mediate virus–endosomal membrane fusion and enable the release of the LASV genome segments into the cytoplasm.

If you have any additional questions please contact us by email: info@virongy.com

Example of results:

Rapid LASV alpha-pseudovirus luciferase transduction of Vero cells (Left): Vero cells were transduced with HA-LASV(Luc) alpha-pseudovirus (with a luciferase reporter). Reporter expression was quantified at 24 hours post-transduction (luciferase assay).

Rapid LASV alpha-pseudovirus GFP transduction of Vero cells (Right): Vero cells were transduced with HA-LASV(GFP) alpha-pseudovirus (with a GFP reporter). Reporter expression was quantified at 24 hours post-transduction (GFP flow cytometry).