Pseudotyped Lentivirus for Betacoronaviruses

Applications

• SARS-CoV, MERS-CoV and SARS-CoV-2 pseudovirus transduction of target cells for viral entry and functional studies
• Anti-SARS-CoV, MERS-CoV and SARS-CoV-2 drug screening
• Anti-SARS-CoV, MERS-CoV and SARS-CoV-2 neutralizing antibody screening

We help our customers to assemble Betacoronavirus pseudotyped lentiviruses for SARS-CoV, MERS-CoV and SARS-CoV-2. These particles carry reporters that can be used for antiviral drug screening or the quantification of neutralizing antibodies. Customers can provide us with the reporter construct of your choice, and we will assemble particles within a week. Please contact us by email: info@virongy.com

We also have several pre-assembled, high-titer particles that are available for your initial testing. Ask about receiving a free sample of our proprietary Infectin, a viral infection enhancer that can greatly promote productive viral infection. Infectin can be used to facilitate the infection of a variety of host cells and can enhance viral infection rates by 3 to 20 fold.

Example of results:

SARS-CoV-2 S protein pseudotyped lentiviral particle transduction of Vero E6 cells (Left):

Vero E6 cells were transduced with SARS-CoV-2-S(Luc) lenti-pseudovirus (with a luciferase reporter) in the presence of CoV-2-PIE. Reporter expression was quantified at 3 days post transduction (luciferase assay).

SARS-CoV S protein pseudotyped lentiviral particle transduction of Vero cells (Right):

Vero cells were transduced with SARS-CoV-S(GFP) lenti-pseudovirus (with a GFP reporter) in the presence of CoV-2-PIE. Reporter expression was quantified at 2 days post transduction (GFP flow cytometry).

SARS-CoV-2 Variant Table: