$50.00$465.00

Viral RNA and DNA Extraction Kits

Virongy’s rapid viral RNA/DNA extraction kits allow for quick and convenient viral genomic isolation for a variety of downstream applications.

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Product Description

Product Description

Rapid Viral RNA and DNA Extraction Kits

Virongy’s rapid viral RNA/DNA extraction kits allow for quick and convenient viral genomic isolation for a variety of downstream applications. These kits provide column-based rapid isolation of high-quality DNA / RNA from fresh or frozen viral preps and viral culture supernatants with a binding capacity of 20ug per column. The kit uses well-established technology for DNA samples from volumes and sizes 20ul – 200ul of the virus. The extracted DNA is suitable for use in many downstream applications, including next-generation sequencing, viral detection, and quantifying viral genome copies.

Key Features:

  • Spin column-based purification
  • Rapid and reliable isolation
  • The binding capacity of 20ug viral DNA / RNA per column
  • Simple, user-friendly protocol
  • High-purity DNA / RNA for qPCR, sequencing, digestion, cloning, and other applications

Example RT-QPCR Results:

Viral RNA was extracted from HA-CoV-2 pseudovirus particles using the Virongy Rapid Viral RNA Extraction kit and quantified by RT-QPCR. 

Viral DNA Extraction Kit Components:

  • DNA Extraction Buffer
  • Washing Buffer
  • Elution Buffer
  • VBI Binding Column
  • Collection Tubes

Viral RNA Extraction Kit Components:

  • RNA Extraction Buffer
  • Washing Buffer
  • Elution Buffer
  • VBI Binding Column
  • Collection Tubes

Viral RNA Extraction Example Protocol: 

1. Thaw virus or virus-like particles on ice.

2. Transfer 350 µL of the thawed virus particle to a 1.5 mL microcentrifuge.

3. Add 350 µL of RNA Extraction Buffer to the tube and mix properly.

4. Transfer the above mix of viruses with RNA Extraction Buffer onto the VBI Binding column.

5. Close the lid and centrifuge at 13,000 rpm for 30 seconds.

6. Discard the flow through.

7. Add 650 µL of Washing Buffer and centrifuge at 13,000 rpm for 30 seconds.

8. Discard the flow through.

9. Close the lid and dry the VBI Binding column by spinning at 13,000 rpm for 30 seconds.

This step will remove all the extra residual liquid remaining in the column.

10. Discard the flow through the tube.

11. Transfer the VBI Binding column to a clean collection microcentrifuge tube and add 30 µL of Elution Buffer to the column.

12. Incubate for 1 minute at room temperature.

13. Spin the VBI Binding column at 13,000 rpm for 1 minute. The elute contains viral RNA. Discard the VBI Binding column.

14. For long-term storage place the extracted viral RNA at -80°C.

 

References