$750.00 – $14,100.00

Rapid Alpha-Pseudoviruses for Influenza A

Name: Rapid Alpha-Pseudoviruses for Influenza A
SKU: 5596
Availability: InStock

Strain

Reporter Gene

Size and Price

Quantity

In stock

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Strain	H1N1--A/Brevig-Mission/1/1918(H1N1), H1N1--A/California/04/2009(H1N1), H1N1--A/Victoria/4897/2022(H1N1), H3N2--A/Darwin/9/2021(H3N2), H5N1--A/Texas/37/2024(H5N1), H5N1--A/Thailand/NBL1/2006(H5N1), H7N9--A/Jiangsu/1/2013(H7N9)
Reporter Gene	Firefly Luciferase
Size and Price	5 x 200 µL ~20 wells/96 well plate, 10 x 200 µL ~40 wells/96 well plate, 5 mL ~100 wells/96 well plate, 10 mL ~200 wells/96 well plate, 20 mL ~400 wells/96 well plate, 5 x 100 µL (Concentrated Only)

SKU:N/A Category:Pseudovirus

Product Description

Rapid Alpha-Pseudovirus for Influenza Virus

Built-to-order alpha-pseudovirus for Influenza viruses (IAV) with the Hemagglutinin (HA), Neuraminidase (NA), Nucleoprotein (NP), Matrix (M1), and Ion Channel (M2).

Applications

IAV pseudovirus transduction of target cells for viral entry and functional studies
Rapid Anti-IAV drug screening
Rapid Anti-IAV neutralizing antibody screening

A novel rapid hybrid alpha-pseudovirus for Influenza virus (Ha-IAV) and other Orthomyxoviridae viruses are now available at Virongy. Ha-IVA particles are pseudoviruses assembled from the structural proteins of the Influenza virus: Hemagglutinin (HA), Neuraminidase (NA), Nucleoprotein (NP), Matrix (M1), and the Ion Channel (M2). They also encapsulate an alphaviral vector for reporter gene expression. The alpha-pseudoviruses are single-cycle viruses with self-replicating RNA for rapid quantification of neutralizing antibodies and entry-inhibiting drugs. These pseudoviruses are BSL-2 safe and ready to use for studying viral entry. Furthermore, we assist our customers in constructing Ha-IVA pseudoviruses at any scale.

Available Strains:

Influenza A/Brevig-Mission/1/1918(H1N1)
Influenza A/California/04/2009(H1N1)
Influenza A/Victoria/4897/2022 (H1N1)
Influenza A/Darwin/9/2021(H3N2)
Influenza A/Thailand/NBL1/2006(H5N1)
Influenza A/Texas/37/2024 (H5N1)
Influenza A/Jiangsu/1/2013(H7N9)

Need a strain not listed here? Ask us about a custom alpha-pseudovirus for your project. For inquiries, please reach out to us via email: info@virongy.com

To enhance your pseudovirus entry, inquire about receiving a complimentary sample of our proprietary Infectin, which significantly enhances productive viral infection in various host cells, thereby boosting the viral infection rate by 3- to 20-fold.

Background:

Alpha-pseudoviruses are a new tool for research and development. Unlike traditional lentivirus or VSV-based systems, which rely on their respective core structural proteins, our alpha-pseudoviruses contain all the authentic structural components of the influenza virus, including HA, NA, M1, M2, and NP proteins. Providing a more biologically accurate and reliable system for studying seasonal, pandemic, and avian influenza strains. Our new system utilizes an alphaviral vector allowing for rapid and robust quantification of reporter signal (luciferase or GFP) in target cells in as little as 6 hours. With its rapid customization capabilities to emerging threats, enhanced safety profile, and robust performance, the alpha-pseudovirus platform sets a new standard for virology research and pandemic preparedness.

Influenza viruses are enveloped with a segmented, single-stranded negative-sense RNA genome, totaling 13.5 Kb in size, divided into 8 segments that code for 12-14 proteins depending on the strain. These segments vary in size from 890 to 2,341 nt. Virions are typically 80-120 nm in diameter and can take on rounded or filamentous shapes. The virus attaches to host cells via sialic acid receptors using the HA protein and enters the cell through clathrin-mediated endocytosis. Acidification of the endosome triggers fusion of the virus membrane with the vesicle membrane, allowing encapsidated RNA segments to migrate to the nucleus. Replication and transcription of genomic segments follow. The viral RNA polymerase (comprising PB1, PB2, and PA subunits) transcribes one mRNA from each genome segment, initiating transcription by cap-snatching from host cell mRNAs. Viral mRNA is then polyadenylated by the viral polymerase through a poly U track stuttering mechanism. Alternative splicing of MP and NS mRNAs yields mRNA encoding M2 and NEP proteins. High levels of M1 protein facilitate genome segment export from the nucleus via the NEP protein, then the virus assembly and budding take place at the plasma membrane.