1. Product Information
Product name: Ha-CoV-2(Luc)
Product No: HaCoV2Luc-01, HaCoV2Luc-10C (10 x concentrated)
Product Batch No: HaCoV2Luc-01-Batch#020721
HaCoV2Luc-10C-Batch#020721
Product Manufacture Date: Feb. 07, 2021
Manufacturer details:
Production Technician Name: D. Yu
Company Address: Virongy Biosciences 11225 Assett Loop STE 111, Manassas, VA 20109
Company Phone Number: (703) 257 5500
Email address: info@virongy.com
2. Quality Control Procedure
Ha-CoV-2 infection Protocol
- Seed 4 x 104 HEK293T(ACE2/TMPRESS2) cells or HEK293T cells into each well of 96-well plates in a total volume of 100 ul cell culture medium (DMEM + 10% FBS + Pen/Strep). Culture overnight at 37°C.
- Remove medium from each well, add 50 ul of Ha-CoV-2(Luc) or 50 ul of concentrated Ha-CoV-2(Luc). Infect for 2 hours.
- Remove virus, add 100 ul freh medium (DMEM + 10% FBS + Pen/Strep). Culture overnight at 37°C for 12-18 hours.
- Remove medium, wash once with PBS, lyse cells with 100 ul of 1 x Cell lysis buffer (Promega).
- Add 100 ul of Luciferease Assay Buffer into each well of 98-well Luciferease Assay Plate. Mix with 30 ul cell lysates.
- Read luciferease activity within 10-15 minutes on a GloMax Discover Microplate Reader.
3. Luciferase Results
Ha-CoV-2 + HEK293T(ACE2/TMPRESS2) cells: 40,480.00 ± 4,925.76 (from 1.3 x 104 infected cells).
10 x Ha-CoV-2 + HEK293T(ACE2/TMPRESS2) cells: 94,366.67 ± 7,902.74 (from 1.3 x 104 infected cells).
HEK293T(ACE2/TMPRESS2) cells only: 42.67 ± 15.28 (from 1.3 x 104 un-infected cells).
Ha-CoV-2 + HEK293T cells: 120.00 ± 2.00 (from 1.3 x 104 infected cells).
HEK293T cells only: 42.7 ± 15.27 (from 1.3 x 104 un-infected cells).
Date of Luciferease Assay: Feb 12, 2021
