Our RCL assay screens lentiviral vector preparations for replication-competent virus using the permissive C8166 T-cell line. Samples are cultured for 14–21 days to allow amplification of any RCL, with detection available via p24 ELISA or RT-qPCR. Positive control virus (R8.71) and single-cycle lentiviral negative controls validate assay performance, ensuring reliable and sensitive detection. Studies can be tailored in duration to meet your project needs, with detailed reporting of results provided.