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$750.00

SARS-CoV-2 Pseudoviral Neutralization Assay Kit

Kit Includes:

  • 5 mL of Pseudovirus Particles
  • 1 Tube of Ready-To-Use Target Cells (5 Million Cells/Tube)
  • 15 mL of VBI Infection Medium
  • 2 mL of Lysis Buffer
  • 3.5 mL of Firefly Luciferase Assay Buffer

Kit Does Not Include Standard Neutralizing Antibody – Available as an Add-On
 RCSNAK-01

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Variant Wild Type, D614G Single Mutation, AY4/BA.1 "Deltacron", Alpha (B.1.1.7), B.1.1.207, B.1.1.298, B.1.2, B.1.258, B.1.494, BA.2, BA.2.12.1, BA.4 and BA.5, BA.4.6, BA.2.75, Beta (B.1.351), BQ.1, BQ.1.1, CH.1.1, Delta (B.1.617.2), Delta Plus (AY.2), Delta Plus (AY4.2), DV.7.1.2, EG.10.1, Epsilon (B.1.427/429), Epsilon (B.1.429), Eta (B.1.525), FL.1.5.1, FL.10.1, GK.1.1, Gamma (P.1), GW.5.1.1, HF.1, HK.3, HV.1, HW.1.1, JB.2.1, JD.1.1, JF.1, JH.2, JN.1, JP.1, Kappa (B.1.617.1), Lambda (C.37), Mu (B.1.621), Omicron (B.1.1.529), XBB.1, XBB.1.5, XBB.1.9.1, XBB.1.16, XBB.1.16.11, XBB.1.41.1, XBB.2.3
N/A

Product Description

Product Description

Pseudoviral Neutralization Assay Kit

Utilizing Our NEW Hybrid Alphavirus Pseudovirus Technology.

Now with ready-to-use cells! No need to culture cells or CO2 incubators. Cells come pre-counted so just thaw and go.

Virongy’s Pseudoviral Neutralization Assay Kit is superior due to the implementation of our newly developed alpha pseudovirus. Alpha pseudoviruses are a newly developed hybrid virus-like particle (VLP) that encapsulates an alphavirus-derived RNA genome for rapid expression of reporter genes (luciferase or GFP) in target cells (Hetrick et al., 2022).

Different from commonly used S protein pseudotyped lenti- or vesicular stomatitis virus (VSV)-pseudoviruses, alpha pseudoviruses are assembled with all of the structural proteins of the target virus. These particles contain a reporter genome derived from an alphavirus-based vector for rapid (4 hours) and robust expression of reporter genes. Alpha pseudoviruses represent a major technological advancement in the development of pseudoviruses and serve as a platform for rapid and robust quantification of neutralization assays, viral mutants, and antiviral drugs (Dabbagh et al., 2021; He et al., 2021).

Virongy offers pre-assembled reporter pseudovirions and variants. We also help customers to perform antiviral drug screening and quantification of neutralizing antibodies. For more information, please contact us by email: info@virongy.com

Alpha pseudovirus major advances

  • A simple protocol that is easier than an ELISA-based method
  • Higher Accuracy than a traditional ELISA-based neutralization assay
  • Faster speed compared to other pseudoviruses– (Maximum reporter expression between 12-18 hours)
  • More robust reporter signal – Alpha pseudoviruses take advantage of an alphavirus vector’s rapid and robust gene expression capacity for reporter expression.
  • High fidelity in particle structure – Alpha pseudoviruses contain all of the virus structural proteins but no structural proteins from other viruses.
  • A strong correlation with the live virus in neutralizing antibody assays. 

Want to read more on the Alpha pseudovirus technology? Click Here

Documents

Neutralizing Antibody Assay Kit – Handbook
Ready To Use Target Cells – MSDS

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